Correct specimen collection procedure. or false, Insert needle through chest wall into pleural. MGM medical college ,mumbai 6. Avoid drawing blood from a hematoma, IVs and catheters Draw slowly when collecting with syringes Avoid drawing the plunger back too forcefully, and avoid frothing of the sample. concerned remains the collection and storage of specimen for laboratory analysis. Normally thin, nonpurulent, whitish or clear, Tests to determine abnormal cells or infection, Culture determines pathogenic microorganisms, Sensitivity determines the antibiotics to which, Swab must advance into nasopharynx to ensure, Ordered to identify organisms growing in sputum, Induration palpable, elevated, hardened area, The elderly freq. Either, Blood or chocolate agar supplemented with, B. pertussis will not grow on media containing, Dont learn this stuff for me at this point. of specimen at the right time, Place the specimen in the appropriate place for, Document/record specimen sent and anything, Delegated to those instructed in skill if, Ordered ac, pc, hs, fasting, before insulin, Antiseptic swab ( allow to dry completely), 3 benefits to measuring progress and results, Allows us to make improvements along the way, Helps us focus on what we value and where we are, Gives info what is happening along the way and. Postural Change in posture from supine to erect or sitting causes a shift in fluid from the intravascular to the interstitial space of about 12%. discuss general procedures, Laboratory Diagnostics, Specimen Collection, and Biosafety Issues - . - Specimen A natural object, part of a collection that is the basic unit of study Labile data (color, smell, etc.) Phone: 352.265.9900 Fax: 877.640.6465 FNA at UF Health Shands Hospital (North Tower): 8:30 a.m. - 3:30 p.m. Cytotech Cell: 352.246.5358 Clipping is a handy way to collect important slides you want to go back to later. role of the nurse. There are selective/differential media for Y. Y. enterocolitica grows as tiny pinpoint lactose, If these plates are incubated at room temperature, Bacteria exhibiting this behavior are further, Staphylococcus aureus, Salmonella enteritidis and, Symptoms of most bacterial food poisoning, Except for the culturing of Salmonella, diagnosis, Gastritis is an inflammation of the stomach or, Upper GI infection symptoms generally include, Bacteria agents responsible for acute toxic food, Gastric biopsies are becoming more common, Helicobacter pylori is the most common cause of, H. pylori produces a very potent urease that can, Urease hydrolyzes urea yielding ammonia ammonia, 80 of UTIs in people ages 2-65 originate from, UTIs are fairly common among females of all ages, Childhood bacteriuria can result in kidney, After age 65 the incidence in men dramatically, Infections of older men are usually associated, The higher incidence of UTIs in hospitalized, UTI pathogens can ascend from the perineum to the, Lower UTIs, urethritis and cystitis, are not, Neonates and children younger than 2 years of age, Symptoms of lower UTIs occur with the same, Upper UTIs present the same symptoms as lower, A majority women over 65 years old with UTIs do, E. coli is the leading cause of community, Other enteric Gram (-) rods that frequently cause, Gram positive bacteria associated with community, Staphylococcus saprophyticus is the leading cause, Other prominent etiologies in hospitalized, Unlike most other clinical specimens, the culture, ?105 CFU/ml of a pure culture from catheterized, Colony counts lt 105 CFU/ml may correlate with, Studies show that symptomatic females with, Two procedures can be used to do urine colony, The calibrated loop method is a much simpler than, In either case there are a few media that are, MacConkey agar or equivalent is always included. The SlideShare family just got bigger. If measles or rubella is suspected, the virus is more likely to be isolated from a paranasal swab. specimen faxes. Arthropod (Tick) for ID. Specimen for detection of viruses should be collected as soon as possible after the appearance of the symptoms that is when the concentration of the virus is at its highest. A, Acute meningitis is a medical emergency - it can, Acute meningitis is commonly caused by only a few. Air drawn in from butterfly or connector tubing. Povidone-iodine wipes/swabs - Used if blood culture is to be drawn. 3. Two serum specimens are required to diagnose an infection serologically. CORD BLOOD specimens that are received with the mother's information on the label can be relabeled with the baby's labels. Date, time and initials of the phlebotomist must be on the label of EACH tube. Scheduled Hours: 40. Blood for antibody testing should be collected as early in the disease as possible and also 5- 10 days later. Avoid repeated freezing and thawing of specimens. % The Phlebotomist/Courier transports laboratory specimens to and from the clinic site and Singing River Health System in accordance with departmental and System procedures and guidelines. Work Shift: Day (United States of America) Clinical Manager, Chemistry Lab. Clamp catheter tubing just below port to allow tubing to fill with urineInsert needle into port and aspirate 5-10 ml.Empty syringe contents into sterile specimen cup and send to lab. Transport to the microbiology laboratory. Once collected samples are recommended to emulsify or mixed properly with viral transport medium (VTM). Can falsely elevate potassium results if drawn before a greentop tube. Select a suitable site for venipuncture Prepare the equipment, the patient and the puncture site. When left unseparated, analytes shift between the cells and the plasma or serum and glucose is consumed. A specimen of nasopharyngeal secretion can also be obtained by using a sterile mucus extractor. %%EOF If unable to collect 3 mL of urine, collect in sterile specimen container (37777) or yellow top tube (104184) and transport urine specimens to the Microbiology Laboratory or refrigerate within 30 minutes. The type of specimen, the appropriate time to obtain the sample, the way of sampling, the storage and transport are critical points in the diagnostic process. endstream endobj 368 0 obj <>stream Oxalate : Interferes with cell membranes, Reasons for a Rejected Specimen No Test Requisition Form (TRF) Test not written on TRF Submitted wrong specimen for test requested Quantity Not Sufficient (QNS) Missing or inadequate identiification Clotted, Avoiding Common Errors in Specimen Collection Insufficient quantity Hemolysis Failure to use the correct container for specimen collection Inaccurate and incomplete patient guidance Failure to label a specimen correctly and to provide all pertinent information Failure to tighten specimen container lids, resulting in leakage and/or contamination of specimen, To prevent a hematoma: Puncture only the uppermost wall of the vein Make sure the needle fully penetrates the upper most wall of the vein. District Microbiologist at (NHM) Ministry of Health Government of India. Throat Swab Culture 2. HUMo0#KI eU(jszH!J~g@ {c?kwhm77o2w"yY(s$\hH>= ?Wh^6MBg|'gYq2Q .1K|B=P*|}6P=iI qSJ\: E-+Uvf{+#/0+Gc*A#Vgl UJy[GGR,1;'IODM 4 0 obj 393 0 obj <>stream Our product offerings include millions of PowerPoint templates, diagrams, animated 3D characters and more. 4. Proper patient identification MANDATORY. Gloves - can be made of latex, rubber, vinyl, etc. COLLECTION AND TRANSPORT Obtain a post-digital rectal exam urine</b> specimen in a urine collection cup (see Instructions Collect the sample in the appropriate container. Do not sell or share my personal information, 1. Our new CrystalGraphics Chart and Diagram Slides for PowerPoint is a collection of over 1000 impressively designed data-driven chart and editable diagram s guaranteed to impress any audience. PowerShow.com is a leading presentation sharing website. We've encountered a problem, please try again. We've updated our privacy policy. Proper preservation ensures a high quality specimen, which increases the quality of information the specimen contains, and increases the value of the maintenance of the specimen. Title: Specimen collection Description: Drainage bag. The newly documented ninety-two titanosaur clutches from Dhar District (Madhya Pradesh State, central India) add further to this extensive data. For certain tests, 24-hour urine samples are collected at home and must be refrigerated during the collection process. Details about immunization and antimicrobial therapy. Date and time of collection. 1.1 Introduction - Specimen Collection The Specimen Collection component consists of two sections, hair collection and nasal swab collection. - Blood- hematuria pH Specific gravity Microscopic examination Stool Specimen Analysis of fecal material can detect OB Pus Ova & Parasites Fecal Vacutainer Tubes, Blood Sample Collection Tubes - BD (Becton Dickinson). Insert needle through chest wall into pleural space. Effects of Exercise: Muscular activity has both transient and longer lasting effects. Urine Specimen Collection and Handling 1. 1. 29 Mai . Specimen Collection and Processing - Free download as Powerpoint Presentation (.ppt / .pptx), PDF File (.pdf), Text File (.txt) or view presentation slides online. major role in success of dot's drug, LABORATORY SPECIMEN COLLECTION & HANDLING - . Collect the sample within 5 days of the onset of the symptoms, and the second sample 5-10 days later. Do not sell or share my personal information, 1. Appropriate specimen selection, collection, and transportation are critical if laboratory results are used to provide information that establishes a diagnosis and successful . Feces specimens are required to diagnose viral gastroenteritis especially that caused by rotaviruses, and to investigate poliomyelitis and other diseases caused by enteroviruses. f47ztf9!oKBt-~Um93V3)7u;ZF-#H1M satisfying kochs second postulate. to comply with the animal welfare ordinance, MLAB 2434 CLINICAL MICROBIOLOGY SUMMER, 2005 CECILE SANDERS & KERI BROPHY - . jitendra kumar pandey,mgm medical college,mumbai. Tap here to review the details. The specimen volume and the suspected organism's nature influence the acceptable transport time. Do you have PowerPoint slides to share? Avoid a probing, traumatic venipuncture Use a larger bore needle (20 gauge) Draw from antecubitalfossa rather than the hand , 2023 SlideServe | Powered By DigitalOfficePro, LABORATORY SPECIMEN COLLECTION & HANDLING, - - - - - - - - - - - - - - - - - - - - - - - - - - - E N D - - - - - - - - - - - - - - - - - - - - - - - - - - -. endstream endobj startxref Mencius said that everyone has a heart of intolerance, a hypertension and diabetic hypertension bp medication what is the fastest way to lower blood sugar heart of concealment, and benevolence. An exception to this rule, throat swabs for S. Since the indigenous microbes are almost always, Also, what is normal in the oropharynx may be, Each URT site can be infected with a variety of, The site most commonly infected is the oropharynx, Pharyngitis is most frequently caused by viruses, Viral phayngitis cultures are not rountinely, The exception to this rule is RSV which can be, Routine cultures of the oropharynx in the U.S.A, S. pyogenes pharyngitis is frequently associated, S. pyogenes can readily be detected by culturing, Some studies have shown that the use of a, SBA containing antibiotics (e.g trimethoprim, A moistened swab on a thin flexible wire is used, It is inserted through one of the nares and slid, When the swab reaches the nasopharyngeal chamber, The procedure is repeated using the same swab in, If C. diptheriae (diphtheria) or Bordetella, Corynebacterium diptheriae (diphtheria) and, However, some individuals are not immune. module 9: myths and facts: tb, hepatitis, The Case for Updating the Medicare Specimen Collection Fee - . Home. More than one routine throat, sputum, feces, However, a single blood culture is not adequate, The number of specimens should be appropriate, A single blood culture is never adequate. SARS-CoV-2: Severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) is an appropriate name for the new coronavirus. Patients Preparation factors ,cont. 2022. Specimen transportation Specimens can be transported from one Laboratory to another or a health facility with no laboratory to one with a laboratory. PowerShow.com is a leading presentation sharing website. dr . 6iaL />~3){aV^3]H>(]~zJ5qw(gw^r#)99GCV0Fu3.SyLh-KpiaOoi}Qv-RJ~i?i)xh>)}m=[T|!1^p>)\#W6,_lgr2 Assess the patient's physical disposition (i.e. What containers are required to collect the specimens? If a feces sample can not be obtained, a rectal swab should be collected and transported in buffered saline. Selective media for the isolation of Salmonella, Selective/differential media routinely used for, The differential agents in these media commonly, Colony color in the case of some media (EMB) is, There is a commercially available selective, Vibrio cholera and V. parahaemolyticus are not, Aeromonas and Pleisiomonas are also diarrhea, To aid in selection of the causative pathogens, Gram Negative (GN) and Selenite broths are two, It takes the normal gut bacteria six hours in GN, Salmonella in selenite, and Salmonella and, After several hours of incubation, the enrichment, Campylobacter jejuni and C. coli are thermophilic, Cefoperazone inhibit enteric Gram negative rods, Various strains of intestinal pathogenic E. coli, Enterohemorrhagic E. coli (EHEC) came to the, Many people became severely ill with painful, EHEC is a potentially deadly strain of E. coli, EHEC is detectable on a selective/differential, Commercially available ELISA tests can be used to, The LGH microbiology lab uses EHEC to report, Diarrhea that develops during a stay in the, Patients who develop diarrhea thee or more days, Nosocomial diarrhea is almost always associated, This antibiotic therapy eradicates a persons, Pseudomembranous colitis is characterized by the, Clostridium difficile is resistant to many, The most common method for detecting C. difficile, Sheeps blood agar will grow the infrequently, There are selective media available for each of, A major characteristic that differentiates, Colonies of oxidase positive Gram negative rods. 1 0 obj The availability of new laboratory techniques for unusual pathogens, makes necessary the review and update of all the steps involved in the processing of the samples. 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